Used APPLIED BIOSYSTEMS / ABI / MDS SCIEX API 4000 Qtrap #9254142 for sale

APPLIED BIOSYSTEMS / ABI / MDS SCIEX API 4000 Qtrap is a triple-quad mass spectrometer, in which three separate components are used to analyze an analyte. First, ions are produced by an atmospheric pressure ionization (APCI) source and then accelerated through a quadrupole lens before being separated in the third component, the quadrupole-time-of-flight (QTOF) mass spectrometer. The ions, after leaving the APCI source, pass through a quadrupole lens, a device that is used to remove interfering ions. Finally, they are directed into the QTOF, which performs the separation based on the time-of-flight technology. QTOF is a type of mass analyzer, which can separate ions based on both their mass-to-charge ratio (m/z) and time-of-flight (TOF). Therefore, the API 4000 is capable of obtaining both highly accurate MS/MS and MS3 spectra without having to add an additional ion-trap component. This makes it ideal for peptide quantitation, characterizing protein post-translational modifications and for the detection of metabolites. The API 4000 is equipped with an APCI source, using a chemical ionization agent of either NH3 or Me-NH3 to ionize compounds. The APCI is capable of widely ranging levels of sensitivity, depending on the ionization parameter. Additionally, the source can be configured to operate in either positive or negative ionization modes and can easily be set up with different ionization parameters. Moreover, the API 4000's APCI source is capable of different functionalization schemes, allowing users to customise the ionization conditions to the sample type. The API 4000's mass analyzer is a three-sector quadrupole-time-of-flight (QTOF) device that is used for the separation of ions. This technique utilises a focalised particle beam of ions and separates them in the time domain based on their arrival time. This means that the ions are focused individually before being accelerated, and then reaching the detector at different times, depending on their m/z. This allows each ion to be measured independently and enables accurate characterization of analytes. The API 4000 additionally has a digital signal processor, a circuit which is used for data acquisition. Data acquisition occurs by using the QTOF mass analyzer, which allows the API 4000 to acquire data with a high degree of accuracy and repeatability. Additionally, the digital signal processor allows the API 4000 to collect data in both single- and multi-channel modes, allowing the user to refine the setting depending on the system requirements. Overall, ABI API 4000 Qtrap combines three components into a high-performance mass spectrometer, capable of peptide quantitation, characterizing protein post-translational modifications and detecting metabolites. Its components include an APCI source, a three-sector QTOF mass analyzer, and a digital signal processor. These components, when combined, allow the API 4000 to acquire data with a high degree of accuracy and repeatability.