Used SIGMA LIFE SCIENCE Tris-Borate-EDTA Buffer #9395397 for sale

SIGMA LIFE SCIENCE Tris-Borate-EDTA Buffer (TBE) is a laboratory chemical and buffer solution used primarily for electrophoretic or chromatographic separations. TBE is used in buffer solutions commonly for agarose gel electrophoresis, Polyacrylamide Gel Electrophoresis (PAGE) and DNA sequencing. It has a neutral pH and an approximate ionic strength of 0.89 so it is especially useful for separating proteins, nucleic acids and other charged molecules. TBE is made up of three main components; Tris (also known as Tris[hydroxymethyl]amino-methane), Borate and EDTA (ethylenediaminetetraacetic acid). Tris is an organic compound found primarily in proteins, and acts as a buffering agent to stabilize the pH of the solution. Borate is a salt that is used to reduce the conductivity and EDTA is a chelating agent used to reduce the interference of metal ions. Typically, a 1.0 Molar concentration of TBE (abbreviated as 1x TBE) is used for electrophoretic and chromatographic separations. To prepare a 1x TBE buffer solution, 105.7 grams of Tris Base, 54.3 grams of Borate and 10.1 grams of EDTA should be added to 1 liter of deionized water and the solution should be brought to a pH of 8.3. In addition, 0.5x and 0.25x TBE concentrations are also commonly used and are generally prepared using the same proportions as 1x TBE but using 50% and 25% of the total amount respectively. TBE can also be used to store DNA because it has significant dissociation constants in comparison to most other buffer systems. To store DNA, a 1x TBE buffer solution should be prepared and the DNA should be manually dissolved in the solution. Once dissolved, the solution should be stored at a temperature range of 4-8°C. The TBE buffer system helps to keep the DNA stable and prevents it from degradation. Aside from electrophoretic and chromatographic separations and storage of DNA, TBE can also be used in other laboratory applications. For example, it can be used in protein and DNA purification as it plays a crucial role in removing ions from mixtures and also increases absorption. In addition, TBE solutions can be used to storage enzymes because its buffering effect keeps the enzyme active and prevents it from denaturing. In conclusion, Tris-Borate-EDTA Buffer (TBE) is an invaluable laboratory tool that has a wide range of applications. It is used mainly for electrophoretic or chromatographic separations and can also be used for storage of DNA, protein and enzyme purification. To prepare a 1x TBE solution, 105.7 grams of Tris Base, 54.3 grams of Borate and 10.1 grams of EDTA should be added to 1 liter of deionized water.