Used APPLIED BIOSYSTEMS SoLiD 4 #9090409 for sale

APPLIED BIOSYSTEMS SoLiD 4 is a laboratory equipment and accessory that provides an efficient and cost effective solution for sample preparation, library construction, and sequencing of DNA and RNA. It is a highly versatile system capable of performing a wide range of molecular biology applications. The system is comprised of four major components that include sequencing by synthesis, sequencing by molecular toeholds, sequencing by hybridization, and sequencing by excision. Together, they provide the user with a comprehensive and integrated solution for analysis of nucleic acids. The sequencing by synthesis process is the cornerstone of SoLiD 4. It uses the exonuclease helicase enzyme to separate single-stranded Complementary DNA (cDNA) molecules and attach fluorescently-labeled nucleotides. The incorporated nucleotides are then washed away, and the remaining strands are analyzed to determine the sequence. Sequencing by molecular toeholds is a single-molecule sequencing process. This process uses a two-step approach to determine the sequence of nucleic acids. In the first step, the molecule is extended with two base pairs of DNA or RNA. In the second step, the hybridized molecules are then scanned with a laser to determine which two nucleotides are attached. Sequencing by hybridization is a method of measuring the relative abundance of nucleotides. This method involves the addition of multiple oligonucleotides, which hybridize with the genomic DNA or cDNA to form a complementary strand. The oligonucleotides are then scanned with a laser to determine the relative abundance of the different nucleotides. Finally, sequencing by excision technology takes advantage of PCR technology and enzymatic cycling to allow sequencing of DNA fragments. In this process, two separate primers are used to amplify the DNA fragment of interest. In the first cycle, an initial template strand is generated. In the next cycles, multiple primer strands are added, which are used to introduce mutations into the initial template. The resulting fragment is then sequenced to yield the desired sequence. Overall, APPLIED BIOSYSTEMS SoLiD 4 is a highly efficient and cost effective solution for sample preparation, library construction, and sequencing of DNA and RNA. It is a powerful technology that provides the user with a comprehensive and integrated solution for analysis of nucleic acids.