Used BD BIOSCIENCES FACS #160931 for sale

BD BIOSCIENCES FACS, or Fluorescence Activated Cell Sorting, is a commonly used laboratory technique that is essential for analyzing the phenotype and function of cells. The process is designed to allow for the separation and purification of cells that contain specific cell surface markers and fluorescent staining patterns. The process uses specialized flow cytometers - instruments that are capable of blending the cell sample with a stream of fluid and aiding the precise detection of individual cells as they pass through a beam of laser light. These lasers allow the precise measurement of fluorescent signals which correspond to the cell properties being measured. The precise measurements captured by the cytometer depend on the laser's intensity and its wavelength settings. FACS machine is also capable of sorting the cells, depending on the fluorescence markers present, into a variety of tubes or dishes. This meticulous method enables researchers to identify and collect cells of the same phenotype or type. The process begins with fluorochrome-conjugated antibodies that bind to antigenic sites on the cellular membrane. A combination of two or more antibodies, known as a panel of antibodies give rise to the fluorescent signal. The magnitude of the signal depends on how much of the fluorescent molecules has been bound to the cell. The mixture of altered cells is then independently sorted and collected as the suspensions enter the laser-beam. The collected cells may then be used for further downstream analysis Using BD BIOSCIENCES FACS equipment and accessories, experts are able to obtain more refined data by separating cells on the basis of multiple parameters; from cell viability to surface markers. Additionally, the company offers many novel technology implementations, including the BDAccel™, which increases event rates up to three times. Essential reagents, such as pre-titrated antibodies, are also offered as part of the service provided by BD BIOSCIENCES. These antibodies are specifically developed to give an optimal signal intensity, which guarantees that the right results will be achieved. In addition to sorting and analyzing cells, FACS machine is also capable of generating a series of useful fluorescence plots and data, which can be used to analyze the vitality, phenotype and genotype of the samples. Furthermore, the collection and storage of cell images are also possible, allowing experts to analyze the collected data and make better-educated decisions. Overall, BD BIOSCIENCES FACS provides unparalleled services in terms of sorting, analyzing and classifying cells. Together with its extensive range of reagents and software applications, the company helps make the research process much more efficient and accurate.