Used BD BIOSCIENCES FACSAria IIu #9253483 for sale
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ID: 9253483
Laser cell sorter
With BD digital analyzer
Includes:
(4) Lasers (375nm, 488nm, 405nm, 633nm)
Blue: (7) PMTs
Red: (3) PMTs
Violet: (3) PMTs
HP Computer
ACDU
HP Monitor
Fluidics cart
Temperature control
LAUDA Chiller
BUFFLALO Whisper aerosol control
Excitation laser lines
Laser powers
Optical configuration
Fluorescence sensitivity:
SPHERO RCP-30-5A Rainbow calibration particles:
Measurement performed at 70 psi and 90 kHz
FITC: (125) Molecules of equivalent soluble fluorochrome (MESF-FITC)
PE: (125) Molecules of equivalent soluble fluorochrome (MESF-PE)
Fluorescence resolution:
Coefficient of Variation (CV)
PI: Area, <3.0%, full G0 / G1 peak for Propidium Iodide (PI)-stained Chicken Erythrocyte Nuclei (CEN)
Hoechst: Area, <3.5%, full G0 / G1 peak for Hoechst-stained CEN
Fluorescence linearity:
Doublet / Singlet ratio
CEN Stained with PI: 1.95-2.05 (Off the 488 nm laser) / Hoechst: 1.95-2.05
(Off the 405 nm laser)
Forward and side scatter sensitivity:
Sensitivity enables separation of fixed platelets from noise
Identification of bacteria
Detection of 0.5-micron beads
Forward and side scatter resolution:
Scatter performance optimized for resolving
Lymphocytes
Monocytes
Granulocytes
Sample acquisition rate:
Maximum acquisition rate (Events per second)
With (12) compensation pairs and (8) parameters: 70000.
BD BIOSCIENCES FACSAria IIu is a flow cytometer that is used for cell sorting and analysis. It is a powerful and versatile instrument that has the ability to analyze up to four colors simultaneously. It can be used for both sample and large populations, as well as for single cell and cell populations. The instrument is equipped with a temperature and pressure controlled cell chamber that is capable of sorting up to 10,000 cells per second. FACSAria IIu utilizes two laser beam-lines to detect fluorescence, scattering, and electronic properties of cells as they pass through the cell chamber. The instrument has the capacity to measure and analyze a wide variety of signals, ranging from small particle identification to cellular signaling and cell cycle measurements. The instrument is also equipped with a number of laboratory accessories that are designed to improve workflow and enhance the functionality of the instrument. These include a buffer delivery station, a sample processor, and a sorting chamber. The buffer delivery station provides a quick, easy method for maintaining ideal sample conditions during the sorting process. The sample processor allows pre-processing and sorting of samples before they are injected into BD BIOSCIENCES FACSAria IIu system. The sorting chamber is designed to reduce background noise, reduce cell clumping, and optimize detection specificity. The chamber provides an ideal environment for the analysis of single cell populations and complex samples. FACSAria IIu is a highly versatile and powerful flow cytometer that is designed to meet the needs of laboratory settings. Its compact size and advanced features make it an attractive choice for laboratories that are working with a variety of cell types. It is capable of providing accurate, real-time results and is easy to use and maintain. As such, it is an ideal choice for a multitude of flow cytometry research applications.
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