Used BECTON DICKINSON BD LSR II #9272063 for sale

BECTON DICKINSON BD LSR II is a versatile, automated flow cytometer and cell analyzer from BECTON DICKINSON (BD) that provides reliable and precise biological data for a wide range of applications. It is designed for accurate and consistent acquisition and analysis of cells in both fluid and solid samples, including cell cultures, tissue sections and whole blood. The equipment is comprised of a high performance flow and cell analysis platform with the ability to simultaneously acquire eight parameters for each sample. The instrument utilizes two lasers that generate the light for the flow cytometry, one 405nm diode laser with adjustable power and an argon laser with a 488nm wavelength. The data acquisition encompasses a flow cell and a set of mirrors and filters that deflect light and cells to the photomultiplier tube (PMT) detectors. BD LSR II uses a solid-state technology and DSP (Digital Signal Processing) to filter and digitize the signal from the photomultipliers. The sample data is collected in a fluorescence intensity range from 0 to 65535 channels. The user-definable compensation algorithm along with the FACSDiva software incorporates scales, shift, multiplicative factors and unit conversions to deliver a normalized data set. The instrument includes two sample injection units, including a standard syringe-based delivery system, which can be used to deliver a diverse range of samples including cell culture, buffy coat, and whole blood samples, for example. It also enables the possibility to perform the 6-color analysis, where up to 6 different fluorescently-labeled antibodies can be used simultaneously. In addition, the unit includes an automated cell sorter that enables the sorting of up to two cell populations at a time, depending on the user's set parameters. The data analysis capabilities of BECTON DICKINSON BD LSR II Instrument are comprehensive and include linear and logarithmic transformations, coupled with 'gate' analysis of cell populations and determination of relative cell numbers. The instrument is also capable of automated doublet discrimination, which is useful in separating dead cells from viable cells, debris from cell subpopulations or for specific cell isolation. The instrument is also equipped with several safety functions, such as a pressure-sensing microfluidic valve machine for sample injection, an electronic safety valve shutoff to ensure absolute containment of the fluorescent material and a cassette airlock for sample insertion and removal. In addition, the tool includes a static protection mode, ensuring optimal protection of fluorescently labelled samples from ambient light. Overall, BD LSR II is a reliable and versatile flow cytometer that offers numerous advantages for researchers. The asset is well-suited for measuring a wide variety of cell parameters, including cell populations and fluorescence intensities, enabling the identification of multiple cell subpopulations in a single sample. Its intuitive design along with the various model safety functions and multicolor detection capabilities make the instrument a highly customizable device for any laboratory.