Used ILLUMINA HiSeq 2500 #9275972 for sale

ILLUMINA HiSeq 2500 is a powerful, industry-standard sequencing platform engineered for high sample throughput, speed and accuracy. This next-generation sequencing (NGS) technology utilizes massively parallel sequencing to sequence DNA fragments simultaneously from up to 24 sample lanes at a time. HiSeq 2500's versatile platform is able to generate a wide range of data, from comprehensive gene expression analysis to comprehensive exome and genome sequencing. It operates at an average output of 250-500 billion bases per run, with short-read lengths of up to 150 bases. ILLUMINA HiSeq 2500 equipment consists of seven modules: the controller, flow cell adaptor, sample pre-processing, cooling modules, control computer, imaging station and data storage. The controller is the main component of HiSeq 2500 system; it is responsible for controlling the sequencing conditions and running sequencing reactions. The accompanying flow cell adapter allows for the simple, automated loading and unloading of flow cells. Pre-processing of samples is done by the dedicated sample pre-processing module, which shears double stranded DNA into fragments and prepares them for re-ligation. The Cooling Module requires liquid nitrogen to maintain a low temperature in order to optimize enzymatic reactions. The Control Computer is the central processor for the entire unit, controlling all sequencing reactions with the help of the Image Station. Lastly, data storage is maintained in the Data Storage module, which can store roughly 20 terabytes in its memory. ILLUMINA HiSeq 2500 offers several different sequencing technologies, including single-read sequencing, paired-end sequencing, and multiplexing & specific amplification. Single-read sequencing is a single-end sequencing technique that sequences fragments in a single direction and offers higher quality data. With paired-end sequencing, sequenced fragments are ligated with different primers to enable higher accuracy in sequence determination. Multiplexing & specific amplification enables sequencing of multiple samples that are either multiplexed into one library or that are all amplified using specific primers. In terms of applications, Genotyping-by-Sequencing (GBS) and Whole-Genome Sequencing are the ideal applications for HiSeq 2500. GBS is used to identify genetic differences between organisms, while Whole-Genome Sequencing can generate a complete map of a genome from start to finish. Additionally, ILLUMINA HiSeq 2500 is useful for ChIP-Seq, CaptureSeq, Bisulfite, Small RNA-Seq, and other sequence-based applications. Overall, HiSeq 2500 provides a powerful NGS machine for researchers and academics alike, offering a vast range of sequencing possibilities and high sample throughputs that can open new doors in research and scientific discovery. Its versatility and scalability make it the ideal platform for a variety of applications, while its easy-to-use tool minimizes the time and effort required to maintain the machine.