Used ILLUMINA NextSeq 500 #293632332 for sale

ILLUMINA NextSeq 500 is a sequencing equipment equipped with uniquely designed sequencing chemistry, reagent mixtures and instrumentation designed to improve performance, significantly shorten time to results, and reduce cost per sample. The system uses long read sequencing chemistry to enable single molecule sequencing for more than 150 bases of data in readable form. NextSeq 500 instrumentation features a short-read ILLUMINA HiSeq™ platform that was carefully modified to enable lower costs and shorter sequencing times. The unit includes advanced imaging and optics, enhanced temperature control, and unified data collection and analysis software that allow for improved reproducibility and performance. ILLUMINA NextSeq 500 machine uses an ILLUMINA HiSeq flow cell, which is critical for proper performance. It is a single-use, closed tool for sample loading and analysis assignments. The flow cell features multiple lanes to block sample run-to-run cross-contamination. NextSeq 500 flow cell vial is filled with sample mixture and then mounted on the instrument. The reagent cocktails included with the flow cell combine the necessary enzymes and buffers that control the DNA polymerase activity required to initiate sequencing. After the flow cell is loaded and sealed, sequencing begins, which is monitored and controlled by instruments. The sequencing chemistry of the asset is designed to yield addition long reads and longer sequencing runs than shorter read technologies. The model's sequencing read lengths are up to 200, 500 and 800bp, depending on the sequencing chemistry used and the application. Its reads are highly accurate, reproducible, and specific for identifying biomarkers and counting alternative splicing events. The sequencing run times are 1-3 hours for single runs and 8-20 hours for multiplexed runs. The instrumentation itself is designed to make the most of ILLUMINA HiSeq flow cell technology for improved imaging optics, temperature control and data collection and analysis. The instrument features 384-channel detection and imaging optics that enhance sample preparation and improve data accuracy and clustering. The equipment also includes advanced temperature control and automated calibration techniques that significantly improve accuracy and reduce assay variability. Finally, ILLUMINA NextSeq 500 system boasts high-performance data collection software that allows for seamless data collection and analysis. The software combines automated parameter settings and flexible control of instrument calibration, data collection, and image analysis. Furthermore, it allows users to define sample groups and perform multiple sample runs. This data collection software also displays read length, droplet size and sequencing uniformity, allowing users to optimize sequencing runs.