Used ROCHE 454 GS FLX #293652525 for sale

ROCHE 454 GS FLX is a sequencing instrument used for sequencing targets from large DNA sample libraries. It has the capacity to unravel both short and long length nucleotide sequences, providing sample-specific information on a larger scale compared to other sequencing platforms. 454 GS FLX equipment consists of three instruments: a pyrosequencer, a thermal cycler, and a bioinformatics processing suite. The pyrosequencer utilizes a process called pyrosequencing, which relies on the detection of the light emitted from the pyrophosphate released by DNA polymerase during the extension of primer-bound templates. By using this method of sequencing, the GS FLX system is capable of sequencing up to 500 base reads with a quality score of 99.5%. The Bioinformatics Processing Suite, which forms the backbone of ROCHE 454 GS FLX unit, offers genome annotation and base pair analysis. This software suite provides a comprehensive data analysis, enabling users to identify and characterize genes, residues, and other genetic elements. In addition, it includes a variety of data management tools that allow users to store their sequencing results internally or externally. The thermal cycler used with the GS FLX machine is capable of sequentially cycling DNA samples in order to optimize the sequencing process. This cycler provides a controlled temperature environment and consists of several components, including a drying oven, a reaction chamber, and multiple probe blocks. Furthermore, the consumable parts of 454 GS FLX tool include the PicoTiter plates and the sequencing reagent mix. The PicoTiterplates come in a variety of sizes, with each containing an array of wells to hold picoliter-sized samples. The sequencing reagent mix contains all the components needed to initiate the polymerase chain reaction, and it also contains an enzyme to facilitate the generation of single-strand DNA molecules from the double-stranded original sample. Overall, ROCHE 454 GS FLX is a powerful sequencing asset that allows users to sequence large sample volumes on a large scale. Its combination of high-throughput capabilities and advanced data analysis makes it a well-suited tool for many laboratory applications.