Used ROCHE 454GS FLX+ #9272713 for sale

ROCHE 454 GS FLX+ is a high-throughput DNA sequencing platform developed by 454 Life Science Corporation, now a part of ROCHE. The GS FLX+ platform produces up to one billion bases of sequence per run, yielding the highest level of coverage. The device uses pyrosequencing technology to sequence DNA fragments over a broad range of sizes from <50 bp to over 5 kb with accurate base-by-base resolution. It is capable of sequencing single-stranded DNA, double-stranded DNA, PCR amplicons, amplicons with adaptors, and synthetic pools of target DNA. The device includes several components, including a reagent cartridge containing both sequencing reagents and the 454 Life Sciences Sequencing enzyme master mix. It also includes a high-resolution, laser-driven CCD camera system that captures fluorescent images and detects the intensity of DNA bases emitted during each step of pyrosequencing. The device is designed for use in laboratories that need fast and accurate DNA sequencing results. It has a very user-friendly graphical interface, and its user-friendly menus allow users to select the most appropriate method for their sequencing needs. ROCHE 454 GS FLX+ has an autonomic sample preparation system, which streamlines the workflow and reduces the possibility of errors. ROCHE 454 GS FLX+ has an automated production mode which automatically optimizes sequencing performance. This can be done by automatically adjusting the flow phase of the reagent mix, the sequencing volume, the rate of sequencing, and the 454 Life Science sequencing enzyme master mix concentrations, all to ensure the highest level of accuracy. The 454 Life Science sequencing enzyme mix that is included with ROCHE 454 GS FLX+ contains four separate enzymes, each of which performs a different function in the sequencing process. These enzymes include Uracil-DNA glycosylase (UDG), Uracil-DNA glycosylase/endonuclease V (UDG/EndV), MutT mimic (MmMt), and Exonuclease VII (ExoVII). ROCHE 454 GS FLX+ is designed to produce up to one billion bases of sequence per run, with an average error rate of 0.5%. This ensures accurate sequencing results and also eliminates the need for additional operations such as PCR or sequencing 7x coverage. The device has an adaptor memory feature for automated adaptor ligation, which enables users to program the adaptors they are using, eliminating the need to manually input them each time. The device also has a data integrity control feature which automatically verifies each step of the sequencing process to ensure that the data is accurate and complete, providing another layer of quality control. Overall, ROCHE 454 GS FLX+ is a reliable, user-friendly platform that can provide accurate and comprehensive DNA sequencing results. Its ease of use, adaptor memory feature, data integrity control, and high output per run combine to make it an ideal choice for high-throughput labs.