Used ROCHE LightCycler 2.0 #9174236 for sale

ROCHE LightCycler 2.0 is a laboratory equipment and accessory used for real-time PCR (polymerase chain reaction). The equipment includes a block-based cycler, a sample processor, a software package, and integrated fluorescence detectors. The LightCycler 2.0 uses fluorescent dyes that enable the detection of target nucleic acids. It is capable of performing dozens of simultaneous reactions in several minutes. The included software is an open source Java application that simplifies multi-step data analysis. The LightCycler 2.0 system is capable of multiplexing with the use of dye labeled primers, allowing monitor multiple targets with a single reaction. It uses dual-additive chemistry which allows for two detection regions for each gene. This allows for more precise gene expression measurements. The unit has several adjustable parameters that allow for user-defined conditions in each experiment. The LightCycler 2.0 sample processing machine is composed of a LightCycler 2.0 96-well Plate reader, a light source, an adjustable temperature block and a pressure hose connecting the block to a syringe. The light source is an adjustable xenon lamp that is used to detect fluorescent signals from amplified products. The temperature block is a Peltier tool that maintains the exact temperature needed for the PCR reactions. The syringe is connected to a sample pump and is used to deliver the PCR master mix into the reaction wells of the plate. The LightCycler 2.0 software package includes several features that allow users to optimize and analyze the results. It allows for automated data processing, basecalling, identification of primer-dimers, melting point determination, and target quantification. Real-time data analysis and graphing features are also available. The package allows for exporting results in the form of graphs or tables, and for noise removal from raw data. Overall, ROCHE LightCycler 2.0 is a powerful and versatile laboratory asset. It is easy to use and allows for accurate gene expression measurements. By connecting the model to the sample processor it allows for automated data analysis and quantification. The open source software package makes multi-step data analysis hassle free. All these features make the LightCycler 2.0 one of the leading systems for real-time PCR.