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THERMO SCIENTIFIC Q Exactive spectrometer is an innovative, high-performance LC-MS/MS instrument designed for robust and reliable analysis of complex sample matrices. It is capable of achieving a resolution of up to 17,500 at 0.7 Hz, which ensures the ability to quickly differentiate between compounds of close similarity. Further, its sensitivity provides lower limit of quantification (LLOQs) down below 1 fg ? s R2 range. Q Exactive spectrometer offers a comprehensive range of application protocols for qualitative and quantitative analysis of large molecules, proteins, and metabolites. Through electrospray ionization (ESI) and Atmospheric Pressure Chemical Ionization (APCI), it provides superior ionization stability and superior detection of complex mixtures. It can also facilitate the generation of fragmentation spectra to facilitate the identification of unknowns through the creation of spectra libraries. The innovative normalized collision energy (NCE) feature of THERMO SCIENTIFIC Q Exactive spectrometer allows optimized quantitative performance and a wide dynamic range due to increased multiplexing capacity. The NCE feature also helps to reduce sample to sample variability in methods between runs, ensuring consistent quantification of analytes having the same abundance. Q Exactive spectrometer is also capable of higher throughput and spectra quality through the Turbo IonSpray source which provides ESI temperatures up to 1400°C. Its quadrupole and hexapole provides refined control over precursor isolation and improved spectral quality in dynamic range. THERMO SCIENTIFIC Q Exactive also offers an unprecedented combination of speed, resolution and sensitivity in a single instrument. Its easy-to-use software package, THERMO SCIENTIFIC Xcalibur, allows users not only to easily create methods, but offers automated peak integration and quantification, as well as report generation. It also has advanced tools to enable complex "post-acquisition" processing of spectra and advanced graphical format report generation. Overall, Q Exactive spectrometer is a powerful and reliable LC-MS/MS tool that is capable of producing high-resolution data and efficient multiplexing. With its superior sensitivity and increased dynamic range, it is ideal for large molecule, metabolite and small molecule analyses. Its wide range of application protocols provides an advanced range of analytical solutions for a variety of analytical challenges.
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